Histology

The Scripps Florida Histology Core (SFHC) is able to provide full histological services as well as technical support for investigators. The lab is equipped with the appropriate ventilated working areas and equipment to prepare, process and stain frozen or paraffin embedded specimens.

Michalina Janiszewska, PhD, holds a slide stained to reveal tumor cells.

About the Histology Core

Tina Van Meter, HT (ASCP), is a nationally certified histotechnician and has many years of experience in various histological methods that are utilized in research laboratories. Her primary focus has been in special stains, immunohistochemistry/immunofluorescence and electron microscopy techniques.

The services are provided to the research community at UF Scripps at a reduced cost.

The Histology Core is located in Building B, Room #244C

Office hours are Monday, Wednesday, Friday, 4 p.m. – 5 p.m.

If you cannot make those hours, please email Tina Van Meter to schedule an appointment.

Resources/Equipment

The Histology Core contains the following instrumentation: 

  • VIP Tissue Processor
  • BIOCARE NxGen Decloaking Chamber
  • Rotary Microtome
  • Staining Center
  • Leica Cryostat
  • Zeiss Axio Microscope/Camera Software
  • Leica Bond-Max Immunostainer
  • Leica Autostainer XL
  • General Data Laser Cassette Marker

Please contact Tina Van Meter at (561) 228-2469 to reserve the Leica Cryostat and Zeiss Axio Microscope.

Special Stains

Ductal cell carcinoma of the breast. Image courtesy NCI

The Core can provide a wide range of special stains. Below is a sample of special stains that we offer. Additional stains are available upon request. Please contact us for a free consultation.

  • Congo Red
  • Crystal Violet
  • Gram Stain
  • Luxol Fast Blue
  • Masson Trichrome
  • Oil Red O
  • Periodic Acid Schiff (PAS)
  • Safronin O

Immunohistochemistry

The Core offers optimization of antibodies and protocols and double and triple staining techniques for immnuohistochemistry/immunofluorescence. Please contact us for a free consultation.

Services

  • Fixation techniques
  • Tissue processing
  • Embedding
  • Paraffin sectioning
  • H&E’s
  • Special stains
  • Immunohistochemistry/immunofluorescence
  • Cryostat embedding and sectioning
  • Decalcification of bone specimens

Pricing

Core users please make an appointment for a free consultation with Tina Van Meter, (561) 228-2469, in advance of a project to discuss tissue preparation, necessary controls and scope of work to maximize the economy and success of the project.

Contacts

Montina Vanmeter

Scientific Manager
Phone: (561) 228-2469
Mailing Address:
120 SCRIPPS WAY
JUPITER FL 33458
Physical Address:
B244c
130 Scripps Way
Histology Core
Jupiter FL 33458

Mayra Caramazana

Lab Admin Coordinator II
Phone: (561) 228-3221
Mailing Address:
130 SCRIPPS WAY # 1B2
JUPITER FL 33458