Biomarkers: Luminex Technology

The Luminex 100/200 technology uses flow cytometry coupled with antibody-conjugated beads uniquely labelled with fluorescence. Up to 100 antigens can be detected in a single sample well of a 96 well plate. The flow cytometric analysis by the xPONENT® software resolves the analyte identity and absolute concentration in the sample based on standard curves included in the assay. Plasma, serum, cell-culture supernatant and cerebrospinal fluid assays are available.

Location, Getting it Done

The Luminex 100/200 is located in B148, Scripps Core Labs Area, Building B Ground Floor. To get started,

The requestor must purchase the Luminex kit of her choice. Magnetic beads preferable. Go to the kit finder page to explore options.

The MILLIPLEX Analyte Quarterly also provides up to date kit inventories. 

Contact Melissa for assistance on choosing your kit and performing the assay. On campus requestors perform their own assay, while the core handles the instrument calibrations, data acquisition and analysis.

External and Scripps CA requestors can ship samples in dry ice to:

Melissa Kazantzis
130 Scripps Way, B110
Jupiter, FL 33458

Pricing

UF Scripps Community: $275 per assay (plus labor if applicable)
External Academic: $275/assay plus applicable indirect costs and labor
External, for-profit: $275/assay plus applicable indirect costs and labor.
*For every additional assay performed on the same day, an additional and cumulative 10% discount on the single assay rate will be applied up to a maximum of four assays in a single day.

  • Autoimmune Autoantibody
  • Immunoglobulin Isotyping
  • Sepsis

Multiplex panels Menu

Immunology

  • Cytokine/Chemokine/Growth Factor
  • Soluble Cytokine Recptor
  • High Sensitivity T Cell
  • CD8+ T Cell
  • Complement
  • MMP
  • TGFβ
  • TIMP
  • Th17

Metabolism and Endocrinology Menu

  • Cardiovascular
  • Apolipoprotein
  • Diabetes
  • Metabolic Hormone
  • IGF Binding Protein
  • Liver Protein
  • Adipokines
  • Adipocyte
  • Myokine
  • Aging
  • Pituitary
  • Bone

Cancer Menu

For detailed information on these panels, go to the Luminex kit finder tool or the Milliplex analyte quarterly.

  • Immuno-Oncology Checkpoint Protein
  • Cancer Autoantibody
  • Angiogenesis/Growth Factor Panel
  • Circulating Cancer Biomarker
  • Cancer/Metastasis Biomarker

Toxicity

  • Liver Injury
  • Kidney Injury

Neurological and Neurodegenerative Disease

  • Neuropeptides/Neurohormones
  • Amyloid β