Hyeryun Choe

Hyeryun Choe, Ph.D.

Professor

Department: SR-IM-CHOE LAB
Business Phone: (561) 228-2440
Business Email: hchoe@ufl.edu

About Hyeryun Choe

Hyeryun Choe has done her graduate studies at Pennsylvania State University and received her Ph.D. degree from the Department of Biochemistry, Molelcular Biology, and Cell Biology in erythrocyte membrane lipid bilayer asymmetry in diseases such as sickle cell anemia. During her postdoctoral training at Beth Israel Hospital and Dana Farber Cancer Institute at Harvard Medical School, her research interests broadened to viruses and the mechanisms with which viruses enter cells and cause diseases. In the decade of 2000, as Assistant and Associate Professor at the Department of Pediatrics, Boston Children’s Hospital, Harvard Medical School, Dr. Choe discovered or co-discovered the HIV-1 coreceptors CCR3 and CCR5, the SARS-CoV receptor ACE2 when it emerged in the winter of 2002, and TFR1 as the receptor for all five South American hemorrhagic fever viruses. Of these, the identification of ACE2 as the receptor for SARS-CoV likely accelerated SARS-CoV-2 research because SARS-CoV-2 also uses ACE2 as its entry receptor. In the following decade after her relocation to the Scripps Research, Dr. Choe, as Associate Professor and Professor, studied the entry mechanisms of newly emerging viruses such as West Nile virus, Zika virus, and SARS-CoV-2. Her passion is to develop or contribute to developing effective vaccines or vaccine alternatives against dengue virus that infects hundreds of millions people and causes diseases in tens of millions a year.

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Research Profile

Viruses enter cells through common underlying mechanisms. Parallel studies of the entry processes of various viruses can therefore highlight differences among them, as well as their similarities. The Choe laboratory studies a wide range of viruses to better understand their entry pathways and the mechanisms of pathogenesis of viral diseases. In doing so, we identified a number of viral receptor and host factors critical for viral infection and pathogenesis. These include: the HIV-1 coreceptor CCR5, and its post-translational modification tyrosine sulfation critical HIV-1 infection; the SARS-CoV receptor angiotensin-converting enzyme 2 (ACE2) and the lysosomal enzyme cathepsin L as an essential target-cell factors for SARS-CoV infection; transferrin receptor 1 (TfR1) as the receptor for New World hemorrhagic fever arenaviruses, and an antibody that inhibits the infection of all five pathogenic New World arenaviruses. We also study how the TIM family of phosphatidylserine receptors promote infections of a wide range of enveloped viruses including filoviruses and flaviviruses such as West Nile, dengue and Zika viruses. More recently, we included adeno-associated virus (AAV), the preferred gene therapy vector, in our study with the goal of improving its transduction efficiency in specific target cells.

Open Researcher and Contributor ID (ORCID)

0000-0003-4538-7176

Publications

2022
Mechanisms of SARS-CoV-2 entry into cells
Nature Reviews Molecular Cell Biology. 23(1):3-20 [DOI] 10.1038/s41580-021-00418-x. [PMID] 34611326.
2021
An Engineered Receptor-Binding Domain Improves the Immunogenicity of Multivalent SARS-CoV-2 Vaccines.
mBio. 12(3) [DOI] 10.1128/mBio.00930-21. [PMID] 33975938.
2021
Clinical Antiviral Drug Arbidol Inhibits Infection by SARS-CoV-2 and Variants through Direct Binding to the Spike Protein.
ACS chemical biology. 16(12):2845-2851 [DOI] 10.1021/acschembio.1c00756. [PMID] 34792325.
2021
Functional importance of the D614G mutation in the SARS-CoV-2 spike protein
Biochemical and Biophysical Research Communications. 538:108-115 [DOI] 10.1016/j.bbrc.2020.11.026. [PMID] 33220921.
2021
How SARS-CoV-2 first adapted in humans.
Science (New York, N.Y.). 372(6541):466-467 [DOI] 10.1126/science.abi4711. [PMID] 33926942.
2021
Hydroxychloroquine-mediated inhibition of SARS-CoV-2 entry is attenuated by TMPRSS2.
PLoS pathogens. 17(1) [DOI] 10.1371/journal.ppat.1009212. [PMID] 33465165.
2021
Mutations derived from horseshoe bat ACE2 orthologs enhance ACE2-Fc neutralization of SARS-CoV-2.
PLoS pathogens. 17(4) [DOI] 10.1371/journal.ppat.1009501. [PMID] 33836016.
2020
A Single Immunization with Nucleoside-Modified mRNA Vaccines Elicits Strong Cellular and Humoral Immune Responses against SARS-CoV-2 in Mice.
Immunity. 53(4):724-732.e7 [DOI] 10.1016/j.immuni.2020.07.019. [PMID] 32783919.
2020
AAV vectors engineered to target insulin receptor greatly enhance intramuscular gene delivery.
Molecular therapy. Methods & clinical development. 19:496-506 [DOI] 10.1016/j.omtm.2020.11.004. [PMID] 33313337.
2020
An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccines.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2020.11.18.388934. [PMID] 33236008.
2020
Mutations from bat ACE2 orthologs markedly enhance ACE2-Fc neutralization of SARS-CoV-2.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2020.06.29.178459. [PMID] 32637954.
2020
Phosphatidylethanolamine and Phosphatidylserine Synergize To Enhance GAS6/AXL-Mediated Virus Infection and Efferocytosis.
Journal of virology. 95(2) [DOI] 10.1128/JVI.02079-20. [PMID] 33115868.
2020
SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity
Nature Communications. 11(1) [DOI] 10.1038/s41467-020-19808-4. [PMID] 33243994.
2020
The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2020.06.12.148726. [PMID] 32587973.
2020
Transferrin receptor 1 is a cellular receptor for human heme-albumin
Communications Biology. 3(1) [DOI] 10.1038/s42003-020-01294-5. [PMID] 33110194.
2019
Reply to Marques and Drexler, “Complex Scenario of Homotypic and Heterotypic Zika Virus Immune Enhancement”.
mBio. 10(5) [DOI] 10.1128/mBio.02073-19. [PMID] 31481388.
2019
Zika Virus-Immune Plasmas from Symptomatic and Asymptomatic Individuals Enhance Zika Pathogenesis in Adult and Pregnant Mice.
mBio. 10(4) [DOI] 10.1128/mBio.00758-19. [PMID] 31266863.
2018
Diverse pathways of escape from all well-characterized VRC01-class broadly neutralizing HIV-1 antibodies
PLOS Pathogens. 14(8) [DOI] 10.1371/journal.ppat.1007238. [PMID] 30125330.
2017
AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses.
Proceedings of the National Academy of Sciences of the United States of America. 114(8):2024-2029 [DOI] 10.1073/pnas.1620558114. [PMID] 28167751.
2016
Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication.
Antimicrobial agents and chemotherapy. 60(4):2195-208 [DOI] 10.1128/AAC.02574-15. [PMID] 26810656.
2016
Zika virus infection during the period of maximal brain growth causes microcephaly and corticospinal neuron apoptosis in wild type mice.
Scientific reports. 6 [DOI] 10.1038/srep34793. [PMID] 27713505.
2015
Virion-associated phosphatidylethanolamine promotes TIM1-mediated infection by Ebola, dengue, and West Nile viruses.
Proceedings of the National Academy of Sciences of the United States of America. 112(47):14682-7 [DOI] 10.1073/pnas.1508095112. [PMID] 26575624.
2014
Human and host species transferrin receptor 1 use by North American arenaviruses.
Journal of virology. 88(16):9418-28 [DOI] 10.1128/JVI.01112-14. [PMID] 24920811.
2013
Dual host-virus arms races shape an essential housekeeping protein.
PLoS biology. 11(5) [DOI] 10.1371/journal.pbio.1001571. [PMID] 23723737.
2013
TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
PLoS pathogens. 9(3) [DOI] 10.1371/journal.ppat.1003232. [PMID] 23555248.
2012
An antibody recognizing the apical domain of human transferrin receptor 1 efficiently inhibits the entry of all new world hemorrhagic Fever arenaviruses.
Journal of virology. 86(7):4024-8 [DOI] 10.1128/JVI.06397-11. [PMID] 22278244.
2012
Enhanced recognition and neutralization of HIV-1 by antibody-derived CCR5-mimetic peptide variants.
Journal of virology. 86(22):12417-21 [DOI] 10.1128/JVI.00967-12. [PMID] 22933279.
2011
A tyrosine-sulfated CCR5-mimetic peptide promotes conformational transitions in the HIV-1 envelope glycoprotein.
Journal of virology. 85(15):7563-71 [DOI] 10.1128/JVI.00630-11. [PMID] 21613393.
2011
Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus.
PLoS pathogens. 7(1) [DOI] 10.1371/journal.ppat.1001258. [PMID] 21253575.
2011
Transferrin receptor 1 in the zoonosis and pathogenesis of New World hemorrhagic fever arenaviruses.
Current opinion in microbiology. 14(4):476-82 [DOI] 10.1016/j.mib.2011.07.014. [PMID] 21807555.
2010
Structural basis for receptor recognition by New World hemorrhagic fever arenaviruses.
Nature structural & molecular biology. 17(4):438-44 [DOI] 10.1038/nsmb.1772. [PMID] 20208545.
2009
A New World primate deficient in tetherin-mediated restriction of human immunodeficiency virus type 1.
Journal of virology. 83(17):8771-80 [DOI] 10.1128/JVI.00112-09. [PMID] 19553332.
2009
Chapter 7. Tyrosine sulfation of HIV-1 coreceptors and other chemokine receptors.
Methods in enzymology. 461:147-70 [DOI] 10.1016/S0076-6879(09)05407-X. [PMID] 19480918.
2009
Host-species transferrin receptor 1 orthologs are cellular receptors for nonpathogenic new world clade B arenaviruses.
PLoS pathogens. 5(4) [DOI] 10.1371/journal.ppat.1000358. [PMID] 19343214.
2009
Mutagenesis and evolution of sulfated antibodies using an expanded genetic code.
Biochemistry. 48(37):8891-8 [DOI] 10.1021/bi9011429. [PMID] 19715291.
2008
Influenza A virus neuraminidase limits viral superinfection.
Journal of virology. 82(10):4834-43 [DOI] 10.1128/JVI.00079-08. [PMID] 18321971.
2008
Protein evolution with an expanded genetic code.
Proceedings of the National Academy of Sciences of the United States of America. 105(46):17688-93 [DOI] 10.1073/pnas.0809543105. [PMID] 19004806.
2008
Receptor determinants of zoonotic transmission of New World hemorrhagic fever arenaviruses.
Proceedings of the National Academy of Sciences of the United States of America. 105(7):2664-9 [DOI] 10.1073/pnas.0709254105. [PMID] 18268337.
2007
Severe acute respiratory syndrome coronavirus entry as a target of antiviral therapies.
Antiviral therapy. 12(4 Pt B):639-50 [PMID] 17944271.
2007
The S proteins of human coronavirus NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2.
Virology. 367(2):367-74 [PMID] 17631932.
2007
Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses.
Nature. 446(7131):92-6 [PMID] 17287727.
2006
A tyrosine-sulfated peptide derived from the heavy-chain CDR3 region of an HIV-1-neutralizing antibody binds gp120 and inhibits HIV-1 infection.
The Journal of biological chemistry. 281(39):28529-35 [PMID] 16849323.
2006
Animal origins of the severe acute respiratory syndrome coronavirus: insight from ACE2-S-protein interactions.
Journal of virology. 80(9):4211-9 [PMID] 16611880.
2006
Conserved receptor-binding domains of Lake Victoria marburgvirus and Zaire ebolavirus bind a common receptor.
The Journal of biological chemistry. 281(23):15951-8 [PMID] 16595665.
2006
Insights from the association of SARS-CoV S-protein with its receptor, ACE2.
Advances in experimental medicine and biology. 581:209-18 [PMID] 17037532.
2006
SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells.
The Journal of biological chemistry. 281(6):3198-203 [PMID] 16339146.
2006
SARS-CoV, but not HCoV-NL63, utilizes cathepsins to infect cells: viral entry.
Advances in experimental medicine and biology. 581:335-8 [PMID] 17037556.
2006
Tyrosine sulfate trapped by amber.
Nature biotechnology. 24(11):1361-2 [PMID] 17093482.
2005
Mapping binding residues in the Plasmodium vivax domain that binds Duffy antigen during red cell invasion.
Molecular microbiology. 55(5):1423-34 [PMID] 15720551.
2005
Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2.
The EMBO journal. 24(8):1634-43 [PMID] 15791205.
2005
Sulphated tyrosines mediate association of chemokines and Plasmodium vivax Duffy binding protein with the Duffy antigen/receptor for chemokines (DARC).
Molecular microbiology. 55(5):1413-22 [PMID] 15720550.
2004
A 193-amino acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 2.
The Journal of biological chemistry. 279(5):3197-201 [PMID] 14670965.
2004
Efficient replication of severe acute respiratory syndrome coronavirus in mouse cells is limited by murine angiotensin-converting enzyme 2.
Journal of virology. 78(20):11429-33 [PMID] 15452268.
2004
N-linked glycosylation in the CXCR4 N-terminus inhibits binding to HIV-1 envelope glycoproteins.
Virology. 324(1):140-50 [PMID] 15183061.
2004
Potent neutralization of severe acute respiratory syndrome (SARS) coronavirus by a human mAb to S1 protein that blocks receptor association.
Proceedings of the National Academy of Sciences of the United States of America. 101(8):2536-41 [PMID] 14983044.
2004
Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2.
Journal of virology. 78(19):10628-35 [PMID] 15367630.
2004
Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120.
Proceedings of the National Academy of Sciences of the United States of America. 101(9):2706-11 [PMID] 14981267.
2003
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus.
Nature. 426(6965):450-4 [PMID] 14647384.
2003
Tyrosine sulfation of human antibodies contributes to recognition of the CCR5 binding region of HIV-1 gp120.
Cell. 114(2):161-70 [PMID] 12887918.
2002
The role of post-translational modifications of the CXCR4 amino terminus in stromal-derived factor 1 alpha association and HIV-1 entry.
The Journal of biological chemistry. 277(33):29484-9 [PMID] 12034737.
2002
Tyrosine-sulfated peptides functionally reconstitute a CCR5 variant lacking a critical amino-terminal region.
The Journal of biological chemistry. 277(43):40397-402 [PMID] 12183462.
The SARS-CoV-2 receptor-binding domain elicits a potent neutralizing response without antibody-dependent enhancement
. [DOI] 10.1101/2020.04.10.036418.

Education

Ph.D. in Microbiology, Biochemistry and Molecular Biology
1984 · Pennsylvania State University
Masters of Science in Microbiology
1980 · Seoul National University
Bachelor's of Science
1977 · Seoul National University

Contact Details

Phones:
Business:
(561) 228-2440
Emails:
Business:
hchoe@ufl.edu
Addresses:
Business Mailing:
Location B207B
120 SCRIPPS WAY BLDG 2B2
JUPITER FL 33458