Kirill Martemyanov

Kirill Martemyanov, Ph.D.

Professor And Chair, Department Of Neuroscience

Department: SR-NEURO-MARTEMYANOV LAB
Business Phone: (561) 228-2000
Business Email: kmartemyanov@ufl.edu

About Kirill Martemyanov

Additional Positions:
Associate Professor, Neuroscience
2011 – 2016 · Scripps Research

Accomplishments

John J. Abel Award
2018 · ASPET
Cogan Award
2014 · Association for Research in Vision and Ophthalmology
McKnight Land-Grant Professorship
2008 ·
European Academy Prize in Biology
1998 ·

Research Profile

The main emphasis of the research in the laboratory is on the fundamental principles that regulate signaling via G protein coupled receptors (GPCR). GPCRs mediate a vast variety of critical biological processes ranging from proliferation and motility to cellular reception and excitability. GPCR signaling pathways are of particular importance for the nervous system function where they control many fundamental processes including excitability, differentiation, sensory perception, and synaptic transmission. Importantly, but not surprisingly, even a subtle imbalance in GPCR signaling often leads to the most profound nervous system disorders ranging from blindness and cognitive problems to grave neurological diseases.

Specifically, our laboratory is interested in understanding the intricate regulatory dynamics of G protein pathways in the following two neuronal systems.

Basal ganglia where G proteins mediate reward behavior and movement coordination underlying not only addictive effects of abused drugs such as opioids and cocaine but also dysfunctions observed in movement disorders such as Parkinson’s disease, Huntington’s disease, Tourette syndrome, and tardive dyskinesia. Retina where G protein signaling systems of primary sensory neurons, photoreceptors, and downstream ON-bipolar cells act in tandem to generate responses to light and synaptically encode them to enable our vision. Dysfunctions in these processes are known to be the leading causes of a range of ocular pathologies including macular degeneration and congenital night blindness. Much of our current efforts are focused on a family of key regulators of GPCR signaling pathways, the Regulator of G protein Signaling (RGS) proteins. RGS proteins constitute a large family of proteins that promote G protein inactivation by facilitating their GTP hydrolysis thus ensuring timely inactivation of the GPCR responses. Many members of the RGS family have been clinically linked to human disease conditions that have been recapitulated in the genetic mouse models. Serving as a central control point in GPCR signaling cascades, RGS proteins hold great promises as targets for the drug development. This brings the major emphasis of our research on elucidating molecular and cellular mechanisms of RGS proteins function in cellular signaling in addition to the efforts to uncover novel regulatory principles.

Research directions in the laboratory include discovery and characterization of novel G protein regulators, delineation of protein-protein interactions, feedback mechanisms, and logistics of signaling pathway organization. In our research, we use a range of multidisciplinary approaches involving identification of components of signaling complexes by proteomics, measurement of protein functional activity by in vitro enzyme kinetics, analysis of synthesis, trafficking and degradation of signaling proteins in cell culture and behavioral characterization of genetic mouse models with altered components of G protein signaling machinery.

Publications

2022
Community guidelines for GPCR ligand bias: IUPHAR review 32.
British journal of pharmacology. [DOI] 10.1111/bph.15811. [PMID] 35106752.
2022
Cryo-EM structure of human GPR158 receptor coupled to the RGS7-Gβ5 signaling complex.
Science (New York, N.Y.). 375(6576):86-91 [DOI] 10.1126/science.abl4732. [PMID] 34793198.
2022
Genetic modeling of GNAO1 disorder delineates mechanisms of Gαo dysfunction.
Human molecular genetics. 31(4):510-522 [DOI] 10.1093/hmg/ddab235. [PMID] 34508586.
2022
Ligand-directed bias of G protein signaling at the dopamine D2 receptor.
Cell chemical biology. 29(2):226-238.e4 [DOI] 10.1016/j.chembiol.2021.07.004. [PMID] 34302750.
2022
Members of the KCTD family are major regulators of cAMP signaling.
Proceedings of the National Academy of Sciences of the United States of America. 119(1) [DOI] 10.1073/pnas.2119237119. [PMID] 34934014.
2022
The role of orphan receptor GPR139 in neuropsychiatric behavior.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 47(4):902-913 [DOI] 10.1038/s41386-021-00962-2. [PMID] 33479510.
2021
Adhesion GPCR Latrophilin 3 regulates synaptic function of cone photoreceptors in a trans-synaptic manner.
Proceedings of the National Academy of Sciences of the United States of America. 118(45) [DOI] 10.1073/pnas.2106694118. [PMID] 34732574.
2021
Diversity of the Gβγ complexes defines spatial and temporal bias of GPCR signaling.
Cell systems. 12(4):324-337.e5 [DOI] 10.1016/j.cels.2021.02.001. [PMID] 33667409.
2021
Extended Phenotyping and Functional Validation Facilitate Diagnosis of a Complex Patient Harboring Genetic Variants in MCCC1 and GNB5 Causing Overlapping Phenotypes.
Genes. 12(9) [DOI] 10.3390/genes12091352. [PMID] 34573334.
2021
Gαo is a major determinant of cAMP signaling in the pathophysiology of movement disorders.
Cell reports. 34(5) [DOI] 10.1016/j.celrep.2021.108718. [PMID] 33535037.
2021
Identification of Potential Modulators of the RGS7/Gβ5/R7BP Complex.
SLAS discovery : advancing life sciences R & D. 26(9):1177-1188 [DOI] 10.1177/24725552211020679. [PMID] 34112017.
2021
Mechanisms of Gβγ Release upon GPCR Activation.
Trends in biochemical sciences. 46(9):703-704 [DOI] 10.1016/j.tibs.2021.05.002. [PMID] 34034924.
2021
Striatal Dopamine Induced ERK Phosphorylation Is Altered in Mouse Models of Monogenic Dystonia.
Movement disorders : official journal of the Movement Disorder Society. 36(5):1147-1157 [DOI] 10.1002/mds.28476. [PMID] 33458877.
2021
Striatal RGS7 Regulates Depression-Related Behaviors and Stress-Induced Reinstatement of Cocaine Conditioned Place Preference.
eNeuro. 8(2) [DOI] 10.1523/ENEURO.0365-20.2020. [PMID] 33402347.
2021
THE CONCISE GUIDE TO PHARMACOLOGY 2021/22: Introduction and Other Protein Targets.
British journal of pharmacology. 178 Suppl 1:S1-S26 [DOI] 10.1111/bph.15537. [PMID] 34529830.
2020
A Global Map of G Protein Signaling Regulation by RGS Proteins.
Cell. 183(2):503-521.e19 [DOI] 10.1016/j.cell.2020.08.052. [PMID] 33007266.
2020
Clarin-1 expression in adult mouse and human retina highlights a role of Müller glia in Usher syndrome.
The Journal of pathology. 250(2):195-204 [DOI] 10.1002/path.5360. [PMID] 31625146.
2020
Free Fatty Acid Receptors as new potential therapeutic target in inflammatory bowel diseases.
Pharmacological research. 152 [DOI] 10.1016/j.phrs.2019.104604. [PMID] 31846762.
2020
GPCR-dependent biasing of GIRK channel signaling dynamics by RGS6 in mouse sinoatrial nodal cells.
Proceedings of the National Academy of Sciences of the United States of America. 117(25):14522-14531 [DOI] 10.1073/pnas.2001270117. [PMID] 32513692.
2020
Haploinsufficiency as a disease mechanism in GNB1-associated neurodevelopmental disorder.
Molecular genetics & genomic medicine. 8(11) [DOI] 10.1002/mgg3.1477. [PMID] 32918542.
2020
Interplay between cell-adhesion molecules governs synaptic wiring of cone photoreceptors.
Proceedings of the National Academy of Sciences of the United States of America. 117(38):23914-23924 [DOI] 10.1073/pnas.2009940117. [PMID] 32879010.
2020
Live cell optical assay for precise characterization of receptors coupling to Gα12.
Basic & clinical pharmacology & toxicology. 126 Suppl 6:88-95 [DOI] 10.1111/bcpt.13228. [PMID] 30916867.
2020
The orphan receptor GPR139 signals via Gq/11 to oppose opioid effects.
The Journal of biological chemistry. 295(31):10822-10830 [DOI] 10.1074/jbc.AC120.014770. [PMID] 32576659.
2019
Allostatic Changes in the cAMP System Drive Opioid-Induced Adaptation in Striatal Dopamine Signaling.
Cell reports. 29(4):946-960.e2 [DOI] 10.1016/j.celrep.2019.09.034. [PMID] 31644915.
2019
Author Correction: RGS7 is recurrently mutated in melanoma and promotes migration and invasion of human cancer cells.
Scientific reports. 9(1) [DOI] 10.1038/s41598-018-37932-6. [PMID] 30850615.
2019
Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures.
Frontiers in cellular neuroscience. 13 [DOI] 10.3389/fncel.2019.00467. [PMID] 31680875.
2019
Beyond the Ligand: Extracellular and Transcellular G Protein-Coupled Receptor Complexes in Physiology and Pharmacology.
Pharmacological reviews. 71(4):503-519 [DOI] 10.1124/pr.119.018044. [PMID] 31515243.
2019
Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3β signaling.
Nature communications. 10(1) [DOI] 10.1038/s41467-019-11575-1. [PMID] 31399584.
2019
ELFN2 is a postsynaptic cell adhesion molecule with essential roles in controlling group III mGluRs in the brain and neuropsychiatric behavior.
Molecular psychiatry. 24(12):1902-1919 [DOI] 10.1038/s41380-019-0512-3. [PMID] 31485013.
2019
Genetic behavioral screen identifies an orphan anti-opioid system.
Science (New York, N.Y.). 365(6459):1267-1273 [DOI] 10.1126/science.aau2078. [PMID] 31416932.
2019
GPR158 in the Visual System: Homeostatic Role in Regulation of Intraocular Pressure.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics. 35(4):203-215 [DOI] 10.1089/jop.2018.0135. [PMID] 30855200.
2019
Homeostatic cAMP regulation by the RGS7 complex controls depression-related behaviors.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 44(3):642-653 [DOI] 10.1038/s41386-018-0238-y. [PMID] 30546127.
2019
Identification of Novel Adenylyl Cyclase 5 (AC5) Signaling Networks in D1 and D2 Medium Spiny Neurons using Bimolecular Fluorescence Complementation Screening.
Cells. 8(11) [DOI] 10.3390/cells8111468. [PMID] 31752385.
2019
NF1-cAMP signaling dissociates cell type-specific contributions of striatal medium spiny neurons to reward valuation and motor control.
PLoS biology. 17(10) [DOI] 10.1371/journal.pbio.3000477. [PMID] 31600280.
2019
Nuclear Receptor Nr4a1 Regulates Striatal Striosome Development and Dopamine D1 Receptor Signaling.
eNeuro. 6(5) [DOI] 10.1523/ENEURO.0305-19.2019. [PMID] 31541002.
2019
The signaling proteins GPR158 and RGS7 modulate excitability of L2/3 pyramidal neurons and control A-type potassium channel in the prelimbic cortex.
The Journal of biological chemistry. 294(35):13145-13157 [DOI] 10.1074/jbc.RA119.007533. [PMID] 31311860.
2018
A High-Throughput Time-Resolved Fluorescence Energy Transfer Assay to Screen for Modulators of RGS7/Gβ5/R7BP Complex.
Assay and drug development technologies. 16(3):150-161 [DOI] 10.1089/adt.2017.839. [PMID] 29658790.
2018
An Input-Specific Orphan Receptor GPR158-HSPG Interaction Organizes Hippocampal Mossy Fiber-CA3 Synapses.
Neuron. 100(1):201-215.e9 [DOI] 10.1016/j.neuron.2018.08.038. [PMID] 30290982.
2018
Correction: The influences of the M2R-GIRK4-RGS6 dependent parasympathetic pathway on electrophysiological properties of the mouse heart.
PloS one. 13(7) [DOI] 10.1371/journal.pone.0200553. [PMID] 29979785.
2018
Expression and relevance of the G protein-gated K+ channel in the mouse ventricle.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-19719-x. [PMID] 29352184.
2018
Improved Scalability of Neuron-Based Phenotypic Screening Assays for Therapeutic Discovery in Neuropsychiatric Disorders.
Molecular neuropsychiatry. 3(3):141-150 [DOI] 10.1159/000481731. [PMID] 29594133.
2018
Inhibitory Signaling to Ion Channels in Hippocampal Neurons Is Differentially Regulated by Alternative Macromolecular Complexes of RGS7.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(46):10002-10015 [DOI] 10.1523/JNEUROSCI.1378-18.2018. [PMID] 30315127.
2018
Interrogating the Spatiotemporal Landscape of Neuromodulatory GPCR Signaling by Real-Time Imaging of cAMP in Intact Neurons and Circuits.
Cell reports. 22(1):255-268 [DOI] 10.1016/j.celrep.2017.12.022. [PMID] 29298426.
2018
Interrogating the Spatiotemporal Landscape of Neuromodulatory GPCR Signaling by Real-Time Imaging of cAMP in Intact Neurons and Circuits.
Cell reports. 24(4):1081-1084 [DOI] 10.1016/j.celrep.2018.07.031. [PMID] 30044975.
2018
LRIT1 Modulates Adaptive Changes in Synaptic Communication of Cone Photoreceptors.
Cell reports. 22(13):3562-3573 [DOI] 10.1016/j.celrep.2018.03.008. [PMID] 29590623.
2018
Making useful gadgets with miniaturized G proteins.
The Journal of biological chemistry. 293(19):7474-7475 [DOI] 10.1074/jbc.H118.002879. [PMID] 29752421.
2018
Molecular Deconvolution Platform to Establish Disease Mechanisms by Surveying GPCR Signaling.
Cell reports. 24(3):557-568.e5 [DOI] 10.1016/j.celrep.2018.06.080. [PMID] 30021154.
2018
Orphan receptor GPR158 controls stress-induced depression.
eLife. 7 [DOI] 10.7554/eLife.33273. [PMID] 29419376.
2018
Pharmacogenomics of GPCR Drug Targets.
Cell. 172(1-2):41-54.e19 [DOI] 10.1016/j.cell.2017.11.033. [PMID] 29249361.
2018
Regional Heterogeneity of D2-Receptor Signaling in the Dorsal Striatum and Nucleus Accumbens.
Neuron. 98(3):575-587.e4 [DOI] 10.1016/j.neuron.2018.03.038. [PMID] 29656874.
2018
RGS7 is recurrently mutated in melanoma and promotes migration and invasion of human cancer cells.
Scientific reports. 8(1) [DOI] 10.1038/s41598-017-18851-4. [PMID] 29330521.
2018
Selective Role of RGS9-2 in Regulating Retrograde Synaptic Signaling of Indirect Pathway Medium Spiny Neurons in Dorsal Striatum.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(32):7120-7131 [DOI] 10.1523/JNEUROSCI.0493-18.2018. [PMID] 30006367.
2018
Structural organization of a major neuronal G protein regulator, the RGS7-Gβ5-R7BP complex.
eLife. 7 [DOI] 10.7554/eLife.42150. [PMID] 30540250.
2018
Synaptic adhesion protein ELFN1 is a selective allosteric modulator of group III metabotropic glutamate receptors in trans.
Proceedings of the National Academy of Sciences of the United States of America. 115(19):5022-5027 [DOI] 10.1073/pnas.1722498115. [PMID] 29686062.
2018
Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor.
Nature communications. 9(1) [DOI] 10.1038/s41467-018-04432-0. [PMID] 29777099.
2018
The influences of the M2R-GIRK4-RGS6 dependent parasympathetic pathway on electrophysiological properties of the mouse heart.
PloS one. 13(4) [DOI] 10.1371/journal.pone.0193798. [PMID] 29668674.
2018
Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors.
Cell reports. 25(1):130-145.e5 [DOI] 10.1016/j.celrep.2018.08.068. [PMID] 30282023.
2017
Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila.
Cell reports. 21(8):2074-2081 [DOI] 10.1016/j.celrep.2017.10.108. [PMID] 29166600.
2017
LGR5 receptor promotes cell-cell adhesion in stem cells and colon cancer cells via the IQGAP1-Rac1 pathway.
The Journal of biological chemistry. 292(36):14989-15001 [DOI] 10.1074/jbc.M117.786798. [PMID] 28739799.
2017
LRIT3 Differentially Affects Connectivity and Synaptic Transmission of Cones to ON- and OFF-Bipolar Cells.
Investigative ophthalmology & visual science. 58(3):1768-1778 [DOI] 10.1167/iovs.16-20745. [PMID] 28334377.
2017
Novel GNB1 mutations disrupt assembly and function of G protein heterotrimers and cause global developmental delay in humans.
Human molecular genetics. 26(6):1078-1086 [DOI] 10.1093/hmg/ddx018. [PMID] 28087732.
2017
The Auxiliary Calcium Channel Subunit α2δ4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors.
Neuron. 93(6):1359-1374.e6 [DOI] 10.1016/j.neuron.2017.02.021. [PMID] 28262416.
2017
The Transduction Cascade in Retinal ON-Bipolar Cells: Signal Processing and Disease.
Annual review of vision science. 3:25-51 [DOI] 10.1146/annurev-vision-102016-061338. [PMID] 28715957.
2016
Association with the Plasma Membrane Is Sufficient for Potentiating Catalytic Activity of Regulators of G Protein Signaling (RGS) Proteins of the R7 Subfamily.
The Journal of biological chemistry. 291(13):7195-204 [DOI] 10.1074/jbc.M115.713446. [PMID] 26811338.
2016
Cellular and Subcellular Localization of the RGS7/Gβ5/R7BP Complex in the Cerebellar Cortex.
Frontiers in neuroanatomy. 10 [PMID] 27965545.
2016
G Protein-Gated K+ Channel Ablation in Forebrain Pyramidal Neurons Selectively Impairs Fear Learning.
Biological psychiatry. 80(10):796-806 [DOI] 10.1016/j.biopsych.2015.10.004. [PMID] 26612516.
2016
GNB5 mutation causes a novel neuropsychiatric disorder featuring attention deficit hyperactivity disorder, severely impaired language development and normal cognition.
Genome biology. 17(1) [PMID] 27677260.
2016
Homozygous GNAL mutation associated with familial childhood-onset generalized dystonia.
Neurology. Genetics. 2(3) [DOI] 10.1212/NXG.0000000000000078. [PMID] 27222887.
2016
Intermolecular Interaction between Anchoring Subunits Specify Subcellular Targeting and Function of RGS Proteins in Retina ON-Bipolar Neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 36(10):2915-25 [DOI] 10.1523/JNEUROSCI.3833-15.2016. [PMID] 26961947.
2016
NF1 Is a Direct G Protein Effector Essential for Opioid Signaling to Ras in the Striatum.
Current biology : CB. 26(22):2992-3003 [DOI] 10.1016/j.cub.2016.09.010. [PMID] 27773571.
2016
Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein.
The Journal of biological chemistry. 291(17):9133-47 [DOI] 10.1074/jbc.M115.694075. [PMID] 26895961.
2016
Regulator of G-Protein Signaling 7 Regulates Reward Behavior by Controlling Opioid Signaling in the Striatum.
Biological psychiatry. 80(3):235-45 [DOI] 10.1016/j.biopsych.2015.07.026. [PMID] 26364547.
2016
RGS proteins as targets in the treatment of intestinal inflammation and visceral pain: New insights and future perspectives.
BioEssays : news and reviews in molecular, cellular and developmental biology. 38(4):344-54 [DOI] 10.1002/bies.201500118. [PMID] 26817719.
2016
Screening of GNAL variants in Brazilian patients with isolated dystonia reveals a novel mutation with partial loss of function.
Journal of neurology. 263(4):665-8 [DOI] 10.1007/s00415-016-8026-2. [PMID] 26810727.
2016
Synergistically acting agonists and antagonists of G protein-coupled receptors prevent photoreceptor cell degeneration.
Science signaling. 9(438) [DOI] 10.1126/scisignal.aag0245. [PMID] 27460988.
2016
The TRPM1 channel in ON-bipolar cells is gated by both the α and the βγ subunits of the G-protein Go.
Scientific reports. 6 [DOI] 10.1038/srep20940. [PMID] 26883481.
2015
Distinct profiles of functional discrimination among G proteins determine the actions of G protein-coupled receptors.
Science signaling. 8(405) [DOI] 10.1126/scisignal.aab4068. [PMID] 26628681.
2015
LRIT3 is essential to localize TRPM1 to the dendritic tips of depolarizing bipolar cells and may play a role in cone synapse formation.
The European journal of neuroscience. 42(3):1966-75 [DOI] 10.1111/ejn.12959. [PMID] 25997951.
2015
Mechanism for Selective Synaptic Wiring of Rod Photoreceptors into the Retinal Circuitry and Its Role in Vision.
Neuron. 87(6):1248-1260 [DOI] 10.1016/j.neuron.2015.09.002. [PMID] 26402607.
2015
Monitoring G Protein Activation in Cells with BRET.
Methods in molecular biology (Clifton, N.J.). 1335:107-13 [DOI] 10.1007/978-1-4939-2914-6_8. [PMID] 26260597.
2015
Orphan Receptor GPR158 Is an Allosteric Modulator of RGS7 Catalytic Activity with an Essential Role in Dictating Its Expression and Localization in the Brain.
The Journal of biological chemistry. 290(22):13622-39 [DOI] 10.1074/jbc.M115.645374. [PMID] 25792749.
2015
Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact visually-guided behavior.
eLife. 4 [DOI] 10.7554/eLife.06358. [PMID] 25879270.
2015
Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling.
eLife. 4 [DOI] 10.7554/eLife.10451. [PMID] 26613416.
2014
G protein signaling in the retina and beyond: the Cogan lecture.
Investigative ophthalmology & visual science. 55(12):8201-7 [DOI] 10.1167/iovs.14-15928. [PMID] 25511392.
2014
GNAL mutations and dystonia–reply.
JAMA neurology. 71(8):1053-4 [DOI] 10.1001/jamaneurol.2014.1509. [PMID] 25111209.
2014
GPR179 is required for high sensitivity of the mGluR6 signaling cascade in depolarizing bipolar cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 34(18):6334-43 [DOI] 10.1523/JNEUROSCI.4044-13.2014. [PMID] 24790204.
2014
Mutations in GNAL: a novel cause of craniocervical dystonia.
JAMA neurology. 71(4):490-4 [DOI] 10.1001/jamaneurol.2013.4677. [PMID] 24535567.
2014
RGS6, but not RGS4, is the dominant regulator of G protein signaling (RGS) modulator of the parasympathetic regulation of mouse heart rate.
The Journal of biological chemistry. 289(4):2440-9 [DOI] 10.1074/jbc.M113.520742. [PMID] 24318880.
2014
RGS7/Gβ5/R7BP complex regulates synaptic plasticity and memory by modulating hippocampal GABABR-GIRK signaling.
eLife. 3 [DOI] 10.7554/eLife.02053. [PMID] 24755289.
2013
Association of Rgs7/Gβ5 complexes with Girk channels and GABAB receptors in hippocampal CA1 pyramidal neurons.
Hippocampus. 23(12):1231-45 [DOI] 10.1002/hipo.22161. [PMID] 23804514.
2013
Essential role of the m2R-RGS6-IKACh pathway in controlling intrinsic heart rate variability.
PloS one. 8(10) [DOI] 10.1371/journal.pone.0076973. [PMID] 24204714.
2013
Macromolecular composition dictates receptor and G protein selectivity of regulator of G protein signaling (RGS) 7 and 9-2 protein complexes in living cells.
The Journal of biological chemistry. 288(35):25129-25142 [DOI] 10.1074/jbc.M113.462283. [PMID] 23857581.
2013
Mutations in GNAL cause primary torsion dystonia.
Nature genetics. 45(1):88-92 [DOI] 10.1038/ng.2496. [PMID] 23222958.
2013
Orphan receptor GPR179 forms macromolecular complexes with components of metabotropic signaling cascade in retina ON-bipolar neurons.
Investigative ophthalmology & visual science. 54(10):7153-61 [DOI] 10.1167/iovs.13-12907. [PMID] 24114537.
2012
GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes.
The Journal of cell biology. 197(6):711-9 [DOI] 10.1083/jcb.201202123. [PMID] 22689652.
2012
Gβ5-RGS complexes are gatekeepers of hyperactivity involved in control of multiple neurotransmitter systems.
Psychopharmacology. 219(3):823-34 [DOI] 10.1007/s00213-011-2409-y. [PMID] 21766168.
2012
R7BP modulates opiate analgesia and tolerance but not withdrawal.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 37(4):1005-12 [DOI] 10.1038/npp.2011.284. [PMID] 22089315.
2012
Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
Proceedings of the National Academy of Sciences of the United States of America. 109(20):7905-10 [DOI] 10.1073/pnas.1202332109. [PMID] 22547806.
2012
The complex of G protein regulator RGS9-2 and Gβ(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum.
Science signaling. 5(239) [DOI] 10.1126/scisignal.2002922. [PMID] 22932702.
2011
Control of striatal signaling by g protein regulators.
Frontiers in neuroanatomy. 5 [DOI] 10.3389/fnana.2011.00049. [PMID] 21852966.
2011
Disruption of the chaperonin containing TCP-1 function affects protein networks essential for rod outer segment morphogenesis and survival.
Molecular & cellular proteomics : MCP. 10(1) [DOI] 10.1074/mcp.M110.000570. [PMID] 20852191.
2011
Interaction of transducin with uncoordinated 119 protein (UNC119): implications for the model of transducin trafficking in rod photoreceptors.
The Journal of biological chemistry. 286(33):28954-28962 [DOI] 10.1074/jbc.M111.268821. [PMID] 21712387.
2011
TRPM1 forms complexes with nyctalopin in vivo and accumulates in postsynaptic compartment of ON-bipolar neurons in mGluR6-dependent manner.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(32):11521-6 [DOI] 10.1523/JNEUROSCI.1682-11.2011. [PMID] 21832182.
2011
Type 5 G protein beta subunit (Gbeta5) controls the interaction of regulator of G protein signaling 9 (RGS9) with membrane anchors.
The Journal of biological chemistry. 286(24):21806-13 [DOI] 10.1074/jbc.M111.241513. [PMID] 21511947.
2010
A conserved protein interaction interface on the type 5 G protein beta subunit controls proteolytic stability and activity of R7 family regulator of G protein signaling proteins.
The Journal of biological chemistry. 285(52):41100-12 [DOI] 10.1074/jbc.M110.163600. [PMID] 20959458.
2010
Gbeta5 recruits R7 RGS proteins to GIRK channels to regulate the timing of neuronal inhibitory signaling.
Nature neuroscience. 13(6):661-3 [DOI] 10.1038/nn.2549. [PMID] 20453851.
2010
Homer 1a gates the induction mechanism for endocannabinoid-mediated synaptic plasticity.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(8):3072-81 [DOI] 10.1523/JNEUROSCI.4603-09.2010. [PMID] 20181604.
2010
Membrane anchor R9AP potentiates GTPase-accelerating protein activity of RGS11 x Gbeta5 complex and accelerates inactivation of the mGluR6-G(o) signaling.
The Journal of biological chemistry. 285(7):4781-7 [DOI] 10.1074/jbc.M109.058511. [PMID] 20007977.
2010
Membrane anchoring subunits specify selective regulation of RGS9·Gbeta5 GAP complex in photoreceptor neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(41):13784-93 [DOI] 10.1523/JNEUROSCI.1191-10.2010. [PMID] 20943919.
2010
Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein.
Journal of neurochemistry. 113(5):1101-12 [DOI] 10.1111/j.1471-4159.2010.06616.x. [PMID] 20100282.
2010
Proteomic identification of Hsc70 as a mediator of RGS9-2 degradation by in vivo interactome analysis.
Journal of proteome research. 9(3):1510-21 [DOI] 10.1021/pr901022m. [PMID] 20095651.
2010
R7BP complexes with RGS9-2 and RGS7 in the striatum differentially control motor learning and locomotor responses to cocaine.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 35(4):1040-50 [DOI] 10.1038/npp.2009.212. [PMID] 20043004.
2010
RGS6/Gβ5 complex accelerates IKACh gating kinetics in atrial myocytes and modulates parasympathetic regulation of heart rate.
Circulation research. 107(11):1350-4 [DOI] 10.1161/CIRCRESAHA.110.224212. [PMID] 20884879.
2009
Biology and functions of the RGS9 isoforms.
Progress in molecular biology and translational science. 86:205-27 [DOI] 10.1016/S1877-1173(09)86007-9. [PMID] 20374717.
2009
Changes in striatal signaling induce remodeling of RGS complexes containing Gbeta5 and R7BP subunits.
Molecular and cellular biology. 29(11):3033-44 [DOI] 10.1128/MCB.01449-08. [PMID] 19332565.
2009
Retina-specific GTPase accelerator RGS11/G beta 5S/R9AP is a constitutive heterotrimer selectively targeted to mGluR6 in ON-bipolar neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(29):9301-13 [DOI] 10.1523/JNEUROSCI.1367-09.2009. [PMID] 19625520.
2009
The R7 RGS protein family: multi-subunit regulators of neuronal G protein signaling.
Cell biochemistry and biophysics. 54(1-3):33-46 [DOI] 10.1007/s12013-009-9052-9. [PMID] 19521673.
2008
A role of RGS proteins in drug addiction.
Biochemical pharmacology. 75(1):76-84 [PMID] 17880927.
2008
Functional comparison of RGS9 splice isoforms in a living cell.
Proceedings of the National Academy of Sciences of the United States of America. 105(52):20988-93 [DOI] 10.1073/pnas.0808941106. [PMID] 19098104.
2008
Human immunodeficiency virus protein Tat induces synapse loss via a reversible process that is distinct from cell death.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(48):12604-13 [DOI] 10.1523/JNEUROSCI.2958-08.2008. [PMID] 19036954.
2008
Targeting of RGS7/Gbeta5 to the dendritic tips of ON-bipolar cells is independent of its association with membrane anchor R7BP.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(41):10443-9 [DOI] 10.1523/JNEUROSCI.3282-08.2008. [PMID] 18842904.
2007
Expression and localization of RGS9-2/G 5/R7BP complex in vivo is set by dynamic control of its constitutive degradation by cellular cysteine proteases.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(51):14117-27 [PMID] 18094251.
2007
Localization and differential interaction of R7 RGS proteins with their membrane anchors R7BP and R9AP in neurons of vertebrate retina.
Molecular and cellular neurosciences. 35(2):311-9 [PMID] 17442586.
2007
The membrane anchor R7BP controls the proteolytic stability of the striatal specific RGS protein, RGS9-2.
The Journal of biological chemistry. 282(7):4772-4781 [DOI] 10.1074/jbc.M610518200. [PMID] 17158100.
2006
Kinetic mechanism of RGS9-1 potentiation by R9AP.
Biochemistry. 45(35):10690-7 [PMID] 16939221.
2006
RGS expression rate-limits recovery of rod photoresponses.
Neuron. 51(4):409-16 [PMID] 16908407.
2006
Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
The Journal of biological chemistry. 281(22):15361-9 [PMID] 16574655.
2006
The N terminus of GTP gamma S-activated transducin alpha-subunit interacts with the C terminus of the cGMP phosphodiesterase gamma-subunit.
The Journal of biological chemistry. 281(10):6194-202 [PMID] 16407279.
2005
R7BP, a novel neuronal protein interacting with RGS proteins of the R7 family.
The Journal of biological chemistry. 280(7):5133-6 [PMID] 15632198.
2005
Sulfhydryl-reactive, cleavable, and radioiodinatable benzophenone photoprobes for study of protein-protein interaction.
Bioconjugate chemistry. 16(3):685-93 [PMID] 15898738.
2004
Absence of the RGS9.Gbeta5 GTPase-activating complex in photoreceptors of the R9AP knockout mouse.
The Journal of biological chemistry. 279(3):1581-4 [PMID] 14625292.
2004
Defects in RGS9 or its anchor protein R9AP in patients with slow photoreceptor deactivation.
Nature. 427(6969):75-8 [PMID] 14702087.
2004
Kinetic approaches to study the function of RGS9 isoforms.
Methods in enzymology. 390:196-209 [PMID] 15488179.
2003
Specificity of G protein-RGS protein recognition is regulated by affinity adapters.
Neuron. 38(6):857-62 [PMID] 12818172.
2003
The DEP domain determines subcellular targeting of the GTPase activating protein RGS9 in vivo.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(32):10175-81 [PMID] 14614075.
2002
Noncatalytic domains of RGS9-1.Gbeta 5L play a decisive role in establishing its substrate specificity.
The Journal of biological chemistry. 277(36):32843-8 [PMID] 12093815.
2002
Specific binding of RGS9-Gbeta 5L to protein anchor in photoreceptor membranes greatly enhances its catalytic activity.
The Journal of biological chemistry. 277(27):24376-81 [PMID] 12006596.
2001
Cell-free production of biologically active polypeptides: application to the synthesis of antibacterial peptide cecropin.
Protein expression and purification. 21(3):456-61 [PMID] 11281721.
2001
Mutations in the G-domain of elongation factor G from Thermus thermophilus affect both its interaction with GTP and fusidic acid.
The Journal of biological chemistry. 276(31):28774-8 [PMID] 11371559.
2001
RGS9-G beta 5 substrate selectivity in photoreceptors. Opposing effects of constituent domains yield high affinity of RGS interaction with the G protein-effector complex.
The Journal of biological chemistry. 276(40):37365-72 [PMID] 11495924.
2000
Domain III of elongation factor G from Thermus thermophilus is essential for induction of GTP hydrolysis on the ribosome.
The Journal of biological chemistry. 275(46):35820-4 [PMID] 10940297.
2000
Extremely thermostable elongation factor G from Aquifex aeolicus: cloning, expression, purification, and characterization in a heterologous translation system.
Protein expression and purification. 18(3):257-61 [PMID] 10733877.
2000
Structure of a mutant EF-G reveals domain III and possibly the fusidic acid binding site.
Journal of molecular biology. 303(4):593-603 [PMID] 11054294.
1999
Domain IV of elongation factor G from Thermus thermophilus is strictly required for translocation.
FEBS letters. 452(3):155-9 [PMID] 10386581.
1998
An intact conformation at the tip of elongation factor G domain IV is functionally important.
FEBS letters. 434(1-2):205-8 [PMID] 9738479.
1998
Increased functional activity of elongation factor G with G16V mutation in the GTP-binding domain.
Biochemistry. Biokhimiia. 63(10):1216-9 [PMID] 9864458.
1997
Direct expression of PCR products in a cell-free transcription/translation system: synthesis of antibacterial peptide cecropin.
FEBS letters. 414(2):268-70 [PMID] 9315699.

Grants

Apr 2022 ACTIVE
Molecular Organization of the First Visual Synapse
Role: Principal Investigator
Funding: NATL INST OF HLTH NEI
Apr 2022 ACTIVE
Orphan Receptors in Regulation of Neuronal G Protein Signaling
Role: Principal Investigator
Funding: NATL INST OF HLTH NIMH
Apr 2022 ACTIVE
Molecular Genetic Mechanisms of Opioid Receptor Signaling
Role: Principal Investigator
Funding: SEATTLE CHILDRENS HOSPITAL via NATL INST OF HLTH NIDA
Apr 2022 ACTIVE
Regulation of Striatal GPCR Signaling
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDA
Apr 2022 ACTIVE
Understanding molecular mechanisms of NF1 function and regulation in the nervous system
Role: Principal Investigator
Funding: US ARMY MED RES ACQUISITION
Apr 2022 ACTIVE
Molecular Basis of Photoreceptor Wiring
Role: Principal Investigator
Funding: NATL INST OF HLTH NEI
Apr 2022 ACTIVE
Trans-synaptic mechanism of retinal synapse formation and function
Role: Principal Investigator
Funding: NATL INST OF HLTH NEI

Education

Ph.D. in Molecular Biology
2000 · Russian Academy of Sciences, Institute of Protein Research
Master's of Science in Molecular Biology
1998 · Pushchino State University
Bachelor's of Science in Biochemistry
1996 · Samara State University

Contact Details

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