Sathyanarayanan V Puthanveettil

Sathyanarayanan V Puthanveettil, Ph.D.

Associate Professor

Department: SR-NEURO-PUTHANVEETTIL LAB
Business Phone: (561) 228-2243
Business Email: sputhanveettil@ufl.edu

About Sathyanarayanan V Puthanveettil

Dr. Puthanveettil graduated from Washington State University where he studied calcium calmodulin dependent protein kinases in the laboratory of Dr. B. W. Poovaiah. He then joined Dr. Eric Kandel’s laboratory at the Howard Hughes Medical Institute, Columbia University, for his post-doctoral research on learning and memory storage.

Related Links:
Additional Positions:
Associate Professor, Department of Neuroscience
2015 – Current · Scripps Research
Faculty member, Kellog School of Science and Technology
2011 – 2011 · The Scripps Research Institute
Adjunct faculty, Department of Biological Sciences
2011 – 2011 · Florida Atlantic University
Adjunct faculty, Department of Biomedical Sciences
2011 – 2011 · Charles E. Schmidt School of Medicine, FAU
Assistant Professor, Department of Neuroscience
2010 – 2014 · Scripps Florida
Associate Research Scientist, Eric Kandel Laboratory
2006 – 2006 · Columbia University Medical Center
HHMI Research Associate/Postdoctoral Research Scientist
2001 – 2006 · Eric Kandel Laboratory, Columbia University Medical Center
Graduate Research Assistant
1996 – 2001 · Poovaiah laboratory, Washington State University, Pullman
Visiting Scientist
1995 – 1996 · Rice Genome Research Program, STAFF Institute
Research Assistant, International Center for Genetic Engineering and Biotechnology (ICGEB)
1994 – 1996 · United Nations Industrial Developmental Organization

Accomplishments

CAREER Award
2015 · National Science Foundation
Whitehall Foundation Award
2012 · Whitehall Foundation
TSRI Nomineee for Alfred Sloan Award, Searle Scholar Award
2011 ·
Young Investigator Award
2011 · Margaret Q. Landenberger Research Foundation
Columbia University Nominee for Blavatnik Award
2010 · New York Academy of Sciences
Cold Spring Harbor Laboratory Registration Waiver
2009 ·
Recognition for work on Kinesin transported RNAs as “HOT TOPIC” for Neuroscience 2009
2009 · Society For Neuroscience (SFN)
Prize for Innovative Research Ideas
2003 · The National Neurofibromatosis Foundation and International Neurofibromatosis Research Foundation
Gordon Research Conference Chair’s Funds for Registration Fees
2000 ·
Travel Award
2000 · Washington State University (WSU) Graduate School
Travel Grant
2000 · American Society for Gravitational and Space Biology (ASGSB)
Cold Spring Harbor Laboratory Registration waiver
1997 ·
National Merit Scholarship
1984-1991 · Department of Education, India

Research Profile

Molecular Logic For The Storage and Maintenance Of Long-Term Memory

The long-term goal of my laboratory is to understand the molecular and cellular basis of memory storage and cognitive disorders. At the cellular level there are two hallmarks of long-term memory storage: experience-dependent activation of gene expression in the nucleus, and local activation of translation at specific synapses.

Previously I have demonstrated in Eric Kandel’s laboratory that activation of fast axonal transport in pre- and post- synaptic neurons during learning is another critical component of long-term memory storage. Activation of molecular transport to synapses is a rate limiting step and coordinates nuclear and synaptic processes during memory storage. The transported cargos include organelles, proteins, mRNAs, and noncoding RNAs.

In an effort to learn what gene products are transported in specific neurons in response to activity, how they are transported, how they are stored for later use, and eventually when and how they are utilized at specific synapses during learning and memory storage, my laboratory uses an integrated approach that combines several high throughput techniques such as genomics and proteomics with electrophysiology, biochemistry, and imaging. A comprehensive understanding of transported cargos will help us in elucidating signaling pathways at the synapse and mechanisms underlying synaptic dysfunction. Animal models such as Aplysia and mice are used to tackle these questions at the cellular and systems levels. In addition, we explore these questions using mouse models for cognitive disorders such as Alzheimer’s and Huntington’s disease.

Publications

2022
Live Imaging and Quantitative Analysis of Organelle Transport in Sensory Neurons of Aplysia Californica.
Methods in molecular biology (Clifton, N.J.). 2431:23-48 [DOI] 10.1007/978-1-0716-1990-2_2. [PMID] 35412270.
2021
Activity-regulated synaptic targeting of lncRNA ADEPTR mediates structural plasticity by localizing Sptn1 and AnkB in dendrites.
Science advances. 7(16) [DOI] 10.1126/sciadv.abf0605. [PMID] 33863727.
2021
Double Duty: Mitotic Kinesins and Their Post-Mitotic Functions in Neurons.
Cells. 10(1) [DOI] 10.3390/cells10010136. [PMID] 33445569.
2021
Molecular motor KIF3B in the prelimbic cortex constrains the consolidation of contextual fear memory.
Molecular brain. 14(1) [DOI] 10.1186/s13041-021-00873-9. [PMID] 34749771.
2021
Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation.
Cell reports. 36(2) [DOI] 10.1016/j.celrep.2021.109369. [PMID] 34260917.
2021
The emerging RNA-centric world of neurobiology.
RNA biology. 18(7):933-935 [DOI] 10.1080/15476286.2021.1930367. [PMID] 34142924.
2020
Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons.
Frontiers in cellular neuroscience. 14 [DOI] 10.3389/fncel.2020.521199. [PMID] 33192305.
2020
Neuron-based high-content assay and screen for CNS active mitotherapeutics.
Science advances. 6(2) [DOI] 10.1126/sciadv.aaw8702. [PMID] 31934620.
2019
Emerging roles for long noncoding RNAs in learning, memory and associated disorders.
Neurobiology of learning and memory. 163 [DOI] 10.1016/j.nlm.2019.107034. [PMID] 31176693.
2019
Synapse Formation Activates a Transcriptional Program for Persistent Enhancement in the Bi-directional Transport of Mitochondria.
Cell reports. 26(3):507-517.e3 [DOI] 10.1016/j.celrep.2018.12.073. [PMID] 30650345.
2018
Improved Scalability of Neuron-Based Phenotypic Screening Assays for Therapeutic Discovery in Neuropsychiatric Disorders.
Molecular neuropsychiatry. 3(3):141-150 [DOI] 10.1159/000481731. [PMID] 29594133.
2018
Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-35634-7. [PMID] 30479371.
2018
Long noncoding RNA GM12371 acts as a transcriptional regulator of synapse function.
Proceedings of the National Academy of Sciences of the United States of America. 115(43):E10197-E10205 [DOI] 10.1073/pnas.1722587115. [PMID] 30297415.
2017
Encoding of contextual fear memory requires de novo proteins in the prelimbic cortex.
Biological psychiatry. Cognitive neuroscience and neuroimaging. 2(2):158-169 [DOI] 10.1016/j.bpsc.2016.10.002. [PMID] 28503670.
2015
Automated measurement of fast mitochondrial transport in neurons.
Frontiers in cellular neuroscience. 9 [DOI] 10.3389/fncel.2015.00435. [PMID] 26578890.
2015
Pharmacological Selectivity Within Class I Histone Deacetylases Predicts Effects on Synaptic Function and Memory Rescue.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 40(10):2307-16 [DOI] 10.1038/npp.2015.93. [PMID] 25837283.
2015
Transcriptome analyses of adult mouse brain reveal enrichment of lncRNAs in specific brain regions and neuronal populations.
Frontiers in cellular neuroscience. 9 [DOI] 10.3389/fncel.2015.00063. [PMID] 25798087.
2014
Aplysia Ganglia preparation for electrophysiological and molecular analyses of single neurons.
Journal of visualized experiments : JoVE. (83) [DOI] 10.3791/51075. [PMID] 24457225.
2014
Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity.
Frontiers in genetics. 5 [DOI] 10.3389/fgene.2014.00084. [PMID] 24795747.
2014
Dissecting mechanisms of brain aging by studying the intrinsic excitability of neurons.
Frontiers in aging neuroscience. 6 [DOI] 10.3389/fnagi.2014.00337. [PMID] 25610394.
2014
High-throughput screening for small molecule modulators of motor protein Kinesin.
Assay and drug development technologies. 12(8):470-80 [DOI] 10.1089/adt.2014.579. [PMID] 25383721.
2014
Huntingtin is critical both pre- and postsynaptically for long-term learning-related synaptic plasticity in Aplysia.
PloS one. 9(7) [DOI] 10.1371/journal.pone.0103004. [PMID] 25054562.
2014
New approach to capture and characterize synaptic proteome.
Proceedings of the National Academy of Sciences of the United States of America. 111(45):16154-9 [DOI] 10.1073/pnas.1401483111. [PMID] 25352669.
2013
A strategy to capture and characterize the synaptic transcriptome.
Proceedings of the National Academy of Sciences of the United States of America. 110(18):7464-9 [DOI] 10.1073/pnas.1304422110. [PMID] 23589870.
2013
Age-associated bidirectional modulation of gene expression in single identified R15 neuron of Aplysia.
BMC genomics. 14 [DOI] 10.1186/1471-2164-14-880. [PMID] 24330282.
2013
Characterization of prion-like conformational changes of the neuronal isoform of Aplysia CPEB.
Nature structural & molecular biology. 20(4):495-501 [DOI] 10.1038/nsmb.2503. [PMID] 23435382.
2013
Decreased response to acetylcholine during aging of aplysia neuron R15.
PloS one. 8(12) [DOI] 10.1371/journal.pone.0084793. [PMID] 24386417.
2013
Genomics and proteomics in solving brain complexity.
Molecular bioSystems. 9(7):1807-21 [DOI] 10.1039/c3mb25391k. [PMID] 23615871.
2013
RNA transport and long-term memory storage.
RNA biology. 10(12):1765-70 [DOI] 10.4161/rna.27391. [PMID] 24356491.
2012
PATHOLOGIES OF AXONAL TRANSPORT IN NEURODEGENERATIVE DISEASES.
Translational neuroscience. 3(4):355-372 [PMID] 23750323.
2012
Spontaneous transmitter release is critical for the induction of long-term and intermediate-term facilitation in Aplysia.
Proceedings of the National Academy of Sciences of the United States of America. 109(23):9131-6 [DOI] 10.1073/pnas.1206914109. [PMID] 22619320.
2012
Spontaneous transmitter release recruits postsynaptic mechanisms of long-term and intermediate-term facilitation in Aplysia.
Proceedings of the National Academy of Sciences of the United States of America. 109(23):9137-42 [DOI] 10.1073/pnas.1206846109. [PMID] 22619333.
2011
Neurexin-neuroligin transsynaptic interaction mediates learning-related synaptic remodeling and long-term facilitation in aplysia.
Neuron. 70(3):468-81 [DOI] 10.1016/j.neuron.2011.03.020. [PMID] 21555073.
2009
Characterization of small RNAs in Aplysia reveals a role for miR-124 in constraining synaptic plasticity through CREB.
Neuron. 63(6):803-17 [DOI] 10.1016/j.neuron.2009.05.029. [PMID] 19778509.
2008
A new component in synaptic plasticity: upregulation of kinesin in the neurons of the gill-withdrawal reflex.
Cell. 135(5):960-73 [DOI] 10.1016/j.cell.2008.11.003. [PMID] 19041756.
2008
Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-term facilitation in Aplysia.
Neuron. 59(6):1024-36 [DOI] 10.1016/j.neuron.2008.07.036. [PMID] 18817739.
2007
Consolidation of learning strategies during spatial working memory task requires protein synthesis in the prefrontal cortex.
Proceedings of the National Academy of Sciences of the United States of America. 104(13):5632-7 [PMID] 17369358.
2006
Neuronal transcriptome of Aplysia: neuronal compartments and circuitry.
Cell. 127(7):1453-67 [PMID] 17190607.
2006
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy.
Nucleic acids research. 34(10):3161-8 [PMID] 16769776.

Grants

Sep 2022 ACTIVE
Novel role of mitotic kinesin KIF11 in structural plasticity and memory
Role: Other
Funding: NATL INST OF HLTH NIMH
Jun 2022 ACTIVE
Assessing effects of manipulation of expression of kinesins in a mouse model of Alzheimer's disease
Role: Principal Investigator
Funding: NATL INST OF HLTH NIA
Apr 2022 ACTIVE
The role of bidirectional transport of lysosome-related organelles in learning and memory storage
Role: Principal Investigator
Funding: NATL INST OF HLTH NIMH
Apr 2022 ACTIVE
Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
Role: Principal Investigator
Funding: NATL INST OF HLTH NIMH
Apr 2022 ACTIVE
Dynein-Dynactin Complex Mediated Retrograde Transport during Long-term Memory Storage
Role: Principal Investigator
Funding: NATL INST OF HLTH NIMH
Apr 2022 ACTIVE
Assessing the role of circRNAs in memory consolidation
Role: Principal Investigator
Funding: NATL INST OF HLTH NIMH
Apr 2022 ACTIVE
Bidirectional Transport of Lysosome Related Organelles during Synapse Maintenance and Plasticity
Role: Principal Investigator
Funding: NATL SCIENCE FOU

Education

Ph.D. in Biochemistry/Biophysics
2001 · Washington State University
Master's of Science in Biotechnology
1994 · Tamil Nadu Agricultural University
Bachelor's of Science in Agriculture
1991 · Kerala Agricultural University

Contact Details

Phones:
Business:
(561) 228-2243
Emails:
Addresses:
Business Mailing:
Location C343
130 SCRIPPS WAY BLDG 3C2
JUPITER FL 33458